The Arab-Kurdish alliance known as the Syrian Democratic Forces (SDF) expelled most of the IS fighters from Manbij last week, but dozens continued to put up tough resistance.
On Friday they withdrew from a northern neighbourhood heading for the IS-held town of Jarabulus along the border with Turkey, taking the captives with them, as the Pentagon said the retreat showed the group was “on the ropes”.
“While withdrawing from a district of Manbij, Daesh (IS) jihadists abducted around 2,000 civilians from Al-Sirb neighbourhood,” said Sherfan Darwish, spokesman for the Manbij Military Council, a key component of the SDF.
“They used these civilians as human shields as they withdrew to Jarabulus, thus preventing us from targeting them,” he said, adding that women and children were among those taken.
The Britain-based Syrian Observatory for Human Rights, which relies on sources inside Syria to cover the war, gave a similar report, saying IS forced around 2,000 civilians into cars it confiscated.
The jihadists, who have suffered a string of losses in Syria and Iraq, have often staged mass kidnappings when they come under pressure to relinquish territory they hold.
“Although fighting in Manbij continues, ISIL is clearly on the ropes. It has lost the centre of Manbij, it has lost control of Manbij,” Pentagon deputy press secretary Gordon Trowbridge said, using another name for the group.
The EU denounced the use of civilians as human shields, saying in a statement that “Daesh (IS) continues to pose a threat to the people of Syria, Iraq, the region as well as to Europe and beyond”.
IS has also used civilians as human shields, booby-trapped cars and carried out suicide bombings to slow advances by their opponents.
Thousands of civilians were held captive by the group in Fallujah, which Iraqi forces recaptured in June after a four-week offensive.
On Friday, the SITE intelligence Group said IS had killed five men in Iraq for smuggling people out of territory it controls.
SDF forces captured Manbij on August 6 but continued to battle pockets of jihadists in parts of the town.
Darwish said the SDF rescued 2,500 civilians who were held captive by IS fighters before they fled and combed Al-Sirb on Friday for any remaining jihadists.
With air support from the US-led coalition, the SDF began its assault on Manbij on May 31, surging into the town itself three weeks later.
But their offensive was slowed by a massive jihadist fightback, before a major push last week saw the alliance seize 90 percent of the town.